XB-IMG-151526
Xenbase Image ID: 151526
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Fig. 9. Tctp regulates axon development via its anti-apoptotic effects. (A-C) Lateral view of DiI-filled retinotectal projections in con-MO-injected or mcl1-MO-injected stage 40 embryos. Dashed lines approximate the boundary of the optic tectum; arrowhead denotes a region of the tract with outgrowth defects; asterisks mark beaded axons, suggestive of degenerating axons; boxed region in C shows axon misprojections into the telencephalon and diencephalon. Panels to the right show enlarged images. The boxed area in C is centred in the ventral optic tract. (D) Mean (±s.e.m.) optic tract width in con-MO-injected and mcl1-MO-injected embryos. C2, **P<0.01, two-way ANOVA. C2-7 denote imaginary, evenly spaced hemi-circumferences centred on the optic chiasm. (E) Relative projection lengths in control and Mcl1 morphant backgrounds. Mean±s.e.m.; n, number of brains analysed; n.s., not significant; Mann–Whitney test. (F) Summary of phenotypic changes in Mcl1 morphant projections (statistical significance determined using Fisher's exact test). (G) Co-delivery of tctp-MO and tctp40-172 mRNA, which encodes a truncated Tctp protein devoid of anti-apoptotic activity, fails to rescue the effects of Tctp depletion on the development of the retinotectal projection. (H) Relative projection lengths in embryos injected with con-MO, tctp-MO or tctp-MO+truncated tctp40-172 mRNA. Mean±s.e.m.; n, number of brains analysed; *P=0.008, Kruskal–Wallis test. (I) Mean (±s.e.m.) optic tract widths. con-MO versus tctp-MO+truncated tctp40-172 mRNA, *P<0.05 (C2), *P<0.05 (C3), *P<0.05 (C4), **P<0.01 (C5), *P<0.05 (C6), two-way ANOVA with Bonferroni correction. Scale bars: 50 μm. Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |