XB-IMG-151646
Xenbase Image ID: 151646
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Fig. 4. Regulation of Xenopus Adprhl1 synthesis revealed by transgenic over-expression.A, B: Eight representative transgenic tadpoles that express human ADPRHL1 protein in their hearts. Each tadpole carries the driver Tg[myl7:Gal4] transgene plus a new integration of a Tg[UAS:human ADPRHL1] responder transgene. Ventral view of stage 44 tadpoles (A) and matching fluorescence image (B) shows anti-Adprhl1 immunocytochemistry (green) and phalloidin stain in the tail (red). C, D: Conversely, recombinant Xenopus Adprhl1 protein does not accumulate in hearts, despite the sibling tadpoles containing the same driver plus a new integration of a Tg[UAS:Xenopus adprhl1] responder transgene. E, F: Detail view of the hearts of each tadpole presented. G, H, K: Significantly, the driver plus the Tg[UAS:Xenopus adprhl1(silent 1-156 bp)] responder containing 36 silent nucleotide changes does produce recombinant Xenopus Adprhl1. I, J, L: Non-transgenic control tadpoles that are siblings to those in (G, H, K). The Adprhl1-peptide antibody binds at aa residues 249-266. H, heart. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |