XB-IMG-151647
Xenbase Image ID: 151647
|
Fig. 5. Branched myofibrils of cells over-expressing a hybrid Adprhl1 protein.A-C: Expression of a N-terminal human-Xenopus hybrid Adprhl1 protein in myocardial cells of a stage 44 transgenic tadpole heart. The tadpole carried the driver Tg[myl7:Gal4] transgene plus a new integration of a Tg[UAS:(FLAG)hum1-94-Xen95-354adprhl1] responder transgene. The entire dissected heart, anterior view, is presented. A: Anti-FLAG immunocytochemistry. B: Anti-Adprhl1. C: Phalloidin actin stain. V, ventricle; OT, outflow tract. Scale bar = 100 μm. d-F: The red square (A) denotes the position of higher magnification images in which cells on the left express recombinant Adprhl1 while those on the right do not. D: Anti-FLAG. E: Phalloidin. F: Channel merge, including DAPI nuclear stain. G: Phalloidin, detail of a 13 μm green square (E) showing actin filament branching (green arrowheads) in cells containing recombinant Adprhl1. Scale bar = 10 μm. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |