XB-IMG-151655
Xenbase Image ID: 151655
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Fig. 6.
Adprhl1 morpholino disrupts myofibril assembly in the forming ventricle.A-D, I-L, Q-T: Dissected hearts from tadpoles injected with Adprhl1-e2i2MO into D-2/4 blastomeres and analyzed at stage 33 (A-D), stage 37 (I-L) and stage 40 (Q-T). E-H, M-P, U-X: Hearts after control morpholino injection into D-2/4 blastomeres. Phalloidin stain of actin filaments (red) and immunocytochemistry for myosin heavy chain (green) are presented. A-H: At the onset of cardiac looping, stage 33, left lateral view of heart tubes (A, B, E, F). Squares denote the position of 105 μm images (C, D, G, H) in the presumptive ventricle region (apex-white arrowheads). Knockdown of adprhl1 prevents the rearrangement of cortical actin and delays myosin production in the ventricle, thus inhibiting formation of striated myofibrils (coloured arrowheads). Additionally, elongation of cardiomyocytes located near the apex fails to occur. I-P: During ventricle outgrowth, stage 37 hearts (I, M) oriented with anterior surface upwards, showing myofibril patterns in the ventricle wall (J-L, N-P). Myofibrils that eventually form after adprhl1 knockdown are disarrayed (white arrowheads) whereas control myofibrils near the apex extend in a perpendicular direction (coloured arrowheads). Q-X: At stage 40, comparable series of hearts (Q, U) and ventricle images (R-T, V-X). Despite increased synthesis, no recovery of myofibril order and direction occurs with the adprhl1 morpholino. Arrowheads mark equivalent sarcomere positions in the paired panels. Scale bars = 100 μm (A, E, I, M, Q, U). Scale bars = 10 μm (all others). Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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