Xenbase Image ID: 151656

Fig. 7. Adprhl1 morpholino affects myofibrils but not cellular membrane structure.A-G: A stage 37 heart, dissected after co-injection of Adprhl1-e2i2MO with membrane-tagged lyn-mCherry RNA into D-2/4 blastomeres. H-N: Heart after control morpholino and lyn-mCherry RNA injection into D-2/4 blastomeres. The anterior surface of the hearts (A, B, H, I) lies upwards and magnified 134 μm images (C-G, J-N) show myofibril and membrane patterns in the ventricle wall. Phalloidin actin (magenta-A, C, H, J), membrane-tagged mCherry (red-B, D, I, K), anti-myosin heavy chain (green-E, L), DAPI (F, M) stains, plus RGB-channel merge (G, N). Panels show reduced myofibril content (arrowheads) in the forming ventricle upon adprhl1 knockdown but no clear change in cellular membrane shape or vesicle structure. Scale bars = 100 μm (A, H). Scale bars = 10 μm (C-G, J-N). White arrowheads, short, splayed myofibrils in morphant. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue myh6.L aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Sometime during NF stage 33 and 34 to NF stage 40 heart myocardium Image source: Published Larger Image Printer Friendly View

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