XB-IMG-151657
Xenbase Image ID: 151657
|
Fig. 8.
Detection of Adprhl1 protein in Xenopus hearts and absence in morpholino injected embryos.A: Western blot detection of Adprhl1 protein in stage 42 hearts and its absence after injection of Adprhl1-e2i2MO into D-2/4 blastomeres. 21 of the small, morphant hearts were harvested compared to 7 controls, while detection of actin was used to normalize the samples. Two Adprhl1 protein bands, 40 and 23 kDa., were identified in control hearts, only. B: Developmental stage series showing the reversed, relative abundance of the two Adprhl1 protein species in older hearts. C: Injection of the second, Adprhl1-i2e3MO produced a similar loss of Adprhl1 protein. D: Adprhl1 detection within embryonic tissues showed the 40 kDa. protein was heart-specific whereas the 23 kDa. species was also found in dissected tail pieces. E: Tadpoles excessively stained for adprhl1 mRNA gave a signal in somite (S) and notochord (N) tissue, suggesting a possible tail source for the 23 kDa. protein (29 hours substrate incubation instead of the typical 2-4 hours). Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Image source: Published Larger Image Printer Friendly View |