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Fig. 9. Rescue of morpholino ventricle beating defect using adprhl1 RNA.A-D: A tadpole injected with Adprhl1-e2i2MO into D-2/4 blastomeres, shown at stage 41. Left lateral (A, B) and ventral view of the dissected heart (C, D). E-H: A sibling tadpole co-injected with Adprhl1-e2i2MO and also adprhl1-HA RNA into D-2/4 blastomeres. The injection masses were 25 ng morpholino and 240 pg RNA. Immunological detection of HA-epitope gave a typical dorsal blastomere injection pattern (F) and signal in the ventricle of the dissected heart (H), which were absent in the tadpole injected solely with the morpholino (B, D). I-L: Comparable images of a sibling control tadpole. Red outlines show the position of the ventricle. The heart of the morpholino-injected tadpole was inactive, causing an oedema to form (A). The ventricle of the rescued tadpole was smaller than controls but actively contracted and no oedema developed (E). In this experiment, none of 53 tadpoles had a beating heart after injection of the morpholino while 31 out of 60 regained ventricular function with co-injection of adprhl1-HA RNA. Scale bars = 100 μm. Ed, oedema; V, ventricle.. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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