Xenbase Image ID: 151660

Fig. 11. Absence of a signal for endogenous Adprhl1 protein in tadpole hearts. Despite the mRNA for adprhl1 being an abundant molecule in embryonic hearts, endogenous levels of Adprhl1 protein are low and are not readily detected by immunocytochemistry. Scarce fluorescent dots observed using the Adprhl1 antibody can be detected in superficial optical sections of all embryonic tissues and are not specific. A-C: Cardiomyocytes in the ventricular region of a looping stage 34 heart tube. A: Anti-Adprhl1, using a far-red channel, fluorescent secondary antibody. B: Phalloidin, anti-myosin heavy chain and DAPI. C: Merge of four channels. D-F: Similar sequence of images of cardiomyocytes from the apex of the ventricle at stage 40. G-I: Stage 44 ventricular cardiomyocytes, this time using a different green channel, fluorescent secondary antibody to attempt Adprhl1 detection. Scale bar (A) = 10 μm and applies to all images. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue adprhl1.L arh2 X. laevis Sometime during NF stage 33 and 34 to NF stage 44 heart myh6.L aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Sometime during NF stage 33 and 34 to NF stage 44 heart cardiac ventricle Image source: Published Larger Image Printer Friendly View

Return to previous page