Xenbase Image ID: 151661

Fig. 12. Binary transgene system for cardiac adprhl1 over-expression. Reproducible cardiac expression of transgenes in founder generation transgenic tadpoles can be achieved using the Gal4/UAS binary system. A: Drawing of transgene DNAs used in the adprhl1 over-expression study. B: Experimental scheme, indicating the stages of development that transgenic tadpoles were analyzed. C: Identification of transgenic tadpoles based on their eye fluorescence. A stage 44 tadpole that carries both the driver Tg[myl7:Gal4, γCrys:eCFP] transgene plus a new integration of a Tg[UAS:Xenopus adprhl1, γCrys:DsRed1] responder transgene. In this double transgenic tadpole, both left and right eyes have widespread cyan and red fluorescence. D: A full list of the transgenes used for over-expression of Adprhl1 proteins, with a summary of their activity and sequence differences compared to Xenopus adprhl1. E: Sequences of the first 282 bp of adprhl1 coding cDNA, for Xenopus and human, plus hybrid (1-52aa) and silent (synonymous) mutation transgenes. Nucleotides that are changed from the Xenopus sequence and present in the human ortholog are coloured red, additional silent changes are green. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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