Xenbase Image ID: 151665

Fig. 16. Expression of recombinant Adprhl1 protein in transgenic hearts.A-F: Expression of Adprhl1 protein in the heart of founder generation transgenic tadpoles. Each carries the Tg[myl7:Gal4] driver plus a new integration of an adprhl1 responder. A:Tg[UAS:(FLAG)human ADPRHL1]. B:Tg[UAS:(FLAG)hum1-94-Xen95-354adprhl1]. C:Tg[UAS:hum1-52-Xen53-354adprhl1]. D:Tg[UAS:(FLAG)Xenopus adprhl1]. E:Tg[UAS:Xenopus adprhl1(silent 1-156bp)]. F: Non-transgenic sibling control heart. Dissected hearts are shown, anterior view, at stage 44 (A-D, F) and stage 42 (E). Cells with a rounded morphology that protrude from the ventricle surface, found in hybrid and silent mutation transgenics are indicated (red arrowheads). The heart (E) represents a transgenic containing large numbers of Xenopus Adprhl1-positive cells and thus shows abnormal cardiac chamber development. A, B, D, F: Anti-FLAG (green) and phalloidin actin stain (red). C, E: Anti-Adprhl1 and phalloidin. Scale bar (A) = 100 μm. V, ventricle; A, atrium; OT, outflow tract; VT ventral transverse skeletal muscle, that was not dissected away from the heart (B). G-L: Adprhl1 protein expression in embryos representing six stable Tg[UAS:adprhl1] transgenic lines. G:Tg[UAS:human ADPRHL1]. H:Tg[UAS:hum1-52-Xen53-354adprhl1]. I:Tg[UAS:Xenopus adprhl1]. J:Tg[UAS:Xenopus adprhl1(silent 1-282bp)]. K:Tg[UAS:(FLAG)human ADPRHL1]. L:Tg[UAS:(FLAG)Xenopus adprhl1]. The developmental stage series highlights the transient nature of Adprhl1 over-expression with the hybrid and silent mutation transgenes. Ventral views, anterior to the left. Image published in: Smith SJ et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page