Xenbase Image ID: 151671

Fig. 5. PKDCC1, a dorsally enriched gene, induced gastrulation defects when ectopically expressed. A) RT-PCR showed that PKDCC1 was first expressed during early gastrulation, and its expression persisted until at least tailbud stages. B) In situ hybridization showed that PKDCC1 was expressed in the organizer at the dorsal lip region in early gastrula embryos. Its expression was then up-regulated in the anterior neural tissues during mid-gastrula to neurula stages. At tailbud stages, PKDCC1 transcripts were seen in the eyes, the lateral plate mesoderm, and the heart primordium. C) PKDCC1 reduced activin-induced animal cap elongation without affecting mesodermal cell fates. D) Ectopic expression of PKDCC1 in early Xenopus embryos induced gastrulation defects, with the resulting embryos displaying reduced body axis, smaller head, and failure in neural tube closure. E) Overexpression of PKDCC1 did not inhibit mesodermal formation in early embryos, as indicated by normal expression of brachyury (Bra), but delayed involution and/or migration of dorsal mesodermal cells marked by chordin (Chd) and goosecoid (Gsc). Image published in: Popov IK et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pkdcc.1.S pkdcc1 X. laevis Sometime during NF stage 10 to NF stage 11 upper blastopore lip dorsal marginal zone involuting marginal zone pkdcc.1.S pkdcc1 X. laevis Throughout NF stage 12 mesoderm involuted dorsal mesoderm pkdcc.1.S pkdcc1 X. laevis Throughout NF stage 16 mesoderm pkdcc.1.S pkdcc1 X. laevis Throughout NF stage 26 lateral plate mesoderm optic vesicle tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 11 mesoderm gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pkdcc.1 + NF31 (morphology) fig.5.d Anatomical Phenotype abnormal bending of anterior-posterior axis abnormal gastrulation abnormal incomplete closing of the neural tube abnormal neural tube closure decreased length of anterior-posterior axis decreased size of the head Xla Wt + pkdcc.1 + NF11 (in situ hybridization) fig.5.e Anatomical Phenotype abnormal gastrulation Expression Phenotype increased amount chrd.1.S expression in upper blastopore lip mislocalised chrd.1.S expression in upper blastopore lip mislocalised gsc.L expression in upper blastopore lip increased amount gsc.L expression in upper blastopore lip Larger Image Printer Friendly View

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