Xenbase Image ID: 151672

Fig. 6. Knockdown of PKDCC1 resulted in gastrulation defects. A) A translational blocking antisense PKDCC1-MO was designed to hybridize to the 5′-UTR sequence of the PKDCC transcript. B) Expression of PKDCC1-MO led to embryos with reduced body axis and smaller head, which were greatly rescued by co-expression of low doses of PKDCC1 RNA that did not contain the 5′-UTR MO-target sequence. C) Knockdown of PKDCC1 resulted in delay in internalization of dorsal mesodermal cells marked by Chd and Gsc. Image published in: Popov IK et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 mesoderm chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 11 mesoderm gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pkdcc.1 MO + NF31 (morphology) fig.6.b Anatomical Phenotype decreased length of anterior-posterior axis decreased size of the head edematous ventral trunk Xla Wt + pkdcc.1 MO + NF11 (in situ hybridization) fig.6.c Anatomical Phenotype abnormal gastrulation Expression Phenotype increased amount chrd.1.S expression in upper blastopore lip mislocalised chrd.1.S expression in upper blastopore lip mislocalised gsc.L expression in upper blastopore lip increased amount gsc.L expression in upper blastopore lip Larger Image Printer Friendly View

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