Xenbase Image ID: 151692

FIG. 2. Stage 33/34 embryos in which a single 32-cell stage blastomere was injected with HRP. Blastomeres from different regions of the embryo (depicted in 2i) were chosen to illustrate the characteristic labeling patterns. The epidermis was removed and the entire embryo histochemically reacted. Progeny of the injected blastomere are brown. Black melanocytes surround the retina, CNS, and dorsal gut; proctodeal and cement gland cells also contain melanin pigment granules. Therefore, these structures may appear pigmented even though they do not contain HRP-labeled progeny. The locations of the organs in the tailbud embryos are depicted in 2j. Bars equal 1 mm. (a) D1.2.1. Labeled progeny populate the cement gland, olfactory placode, retina, lens, cranial ganglia, otocyst, brain and spinal cord, dorsal head mesenchyme, branchial arches, heart, head somites, central trunk somites, ventral gut, and liver. Note how different the distribution of labeled cells is compared to that of a neighboring cell shown in 2e. (b) D1.1.2. Labeled progeny populate the retina, cranial ganglia, brain and spinal cord, dorsal head mesenchyme, branchial arches, heart, notochord, head somites, and central trunk somites. The progeny distribution is distinct from that of the other dorsal animal pole blastomere (2a) and very different from the other tier 2 blastomere (2f). (c) D2.1.1. Labeled progeny in this specimen populate one branchial arch, heart, liver, and gut. The distribution of its progeny is different from that of a neighbor cell in the same tier (Zd). (d) D2.2.1. Labeled progeny in this specimen populate spinal cord, trunk somites, nephrotome, lateral plate, and gut. Many of the labeled structures lie deep to other labeled structures and thus are not distinctly identifiable at the focal plane of the photomicrograph (e.g., spinal cord, archenteron roof, nephrotome). The distribution of the progeny of D2.2.1 is distinctly different from those of its two tier 4 neighbors (2~ and 2h). (e) V1.2.1. Labeled progeny in this specimen populate the cement gland, olfactory placode, lens, three distinct cranial ganglia (rostra1 to caudal: V, VII, IX/X), otocyst, dorsal brain, dorsal spinal cord, branchial arches, heart, a few cells in the trunk somites, nephrotome, rostra1 lateral plate, liver, and ventral gut. The distribution of its progeny is very different from that of its neighbor (2a) and from that of the other ventral animal pole blastomere (2f). (f) V1.1.2. Labeled progeny in this specimen populate caudal trunk and tail structures. Those that can be clearly identified in the photomicrograph are dorsal and ventral trunk somite, lateral plate mesoderm, ventral gut, and proctodeum. (g) V2.2.2. Labeled progeny in this specimen populate cranial ganglia, otocyst, dorsal CNS, dorsal head mesenchyme, branchial arches, somites, nephrotome, lateral plate, gut, and proctodeum. The distribution of its progeny is distinct from that of its sister cell (2h). (h) Labeled progeny in this specimen can most clearly be identified in ventral tail somite, nephrotome, lateral plate, gut, and proctodeum. Image published in: Moody SA (1987) Copyright © 1987. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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