Xenbase Image ID: 151761

Fig. 4. Sdc4 mediates Fn1 matrix deposition in the foregut (A–C) Confocal Fn1 immunostaining in the foregut of control-MO, Sdc4-MO and Fzd7-MO injected embryos at stage NF19. In controls peri-cellular Fn1 is observed in the foregut around the closing blastocoel and two Fn1 matrix layers: one between the foregut endoderm (en) and cardiac mesoderm, (m) and the other layer between foregut mesoderm (m) and ectoderm (ec). The Fn1 layer between the endoderm and mesoderm is largely absent (arrows) in Sdc4-depleted embryos. (D) Western blot analysis of dissected NF19 foreguts tissue shows that total Fn1 protein levels are unchanged in Sdc4-depleted embryos as compared to controls. Image published in: Zhang Z et al. (2016) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Experiment + Assay Source Phenotypes and Disease Xla Wt + sdc4 MO + NF19 (immunohistochemistry) Fig 4 B Expression Phenotype decreased amount fn1.L expression in extracellular matrix Larger Image Printer Friendly View

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