Xenbase Image ID: 151764

Fig. 7. The Sdc4 PDZ-binding domain is dispensable for Fn1 matrix assembly and BMP signaling, but required cell morphology (A–D) Confocal Fn1 immunostaining in the foregut region of bisected embryos at stage NF19. (E–H) Confocal immunostaining of pSmad1/5/8 at NF19. Quantification showed in Supplemental Fig. S7 (I–L) Confocal immunostaining of cytoskeletal β-catenin showing foregut cell morphology at NF19. (M–P) In situ hybridization with hhex. Numbers in the figure show the number of embryos with representative phenotype on the left versus total embryos examined on the right. Image published in: Zhang Z et al. (2016) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Experiment + Assay Source Phenotypes and Disease Xla Wt + sdc4 MO + NF19 (immunohistochemistry) Fig 7 B Expression Phenotype decreased amount fn1.L expression in extracellular matrix absent smad1.L expression in foregut endoderm Xla Wt + sdc4 MO + NF19 (immunohistochemistry) Fig 7 J Anatomical Phenotype abnormal endoderm morphology abnormal regulation of cell size Xla Wt + sdc4 MO + NF19 (in situ hybridization) Fig 7 N Expression Phenotype decreased amount hhex.L expression in foregut endoderm Larger Image Printer Friendly View

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