Xenbase Image ID: 151777

Fig. 2. prdm12 is dependent on RA signaling and Pax6, and is repressed by Dbx1 and Nkx6. (A) RT-PCR analysis of the expression of the indicated genes in animal caps isolated from wild-type or noggin-injected (100 pg/blastomere) embryos, cultivated in the presence or absence of RA. Expression levels were compared with the expression level of uninjected caps, which was defined as 1. (B,C,F,G) prdm12 expression in Xenopus neurula-stage embryos injected unilaterally at the 2-cell stage with the indicated mRNA (500 pg/blastomere). (D,E,H,K). prdm12 is downregulated in the spinal cord of E12.5 pax6Sey/Sey mutants and is expanded in dbx1lacZ/lacZ and nkx6-1−/− embryos (brackets) (n≥2 for all mutant lines). IS, injected side. Image published in: Thélie A et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue prdm12.L LOC108699953, pfm9 X. laevis Throughout NF stage 13 neural plate posterior placodal area Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pax6 MO + NF13 (in situ hybridization) Fig.2.c Expression Phenotype decreased amount prdm12.L expression in chordal neural plate Xla Wt + dbx1 + NF13 (in situ hybridization) Fig.2.f Expression Phenotype decreased amount prdm12.L expression in chordal neural plate Xla Wt + nkx6-1 + NF13 (in situ hybridization) Fig.2.g Expression Phenotype decreased amount prdm12.L expression in chordal neural plate decreased amount prdm12.L expression in cranial placode Larger Image Printer Friendly View

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