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XB-IMG-151779

Xenbase Image ID: 151779


Fig. 4. Xenopus and mouse Prdm12, but not amphioxus Prdm12, downregulate dbx1, nkx6-1 and nkx6-2, and induces en1 in Xenopus. (A-F) Transverse sections at the neural tube of stage 32 embryos injected unilaterally with mPrdm12 mRNA (200 pg/blastomere) and hybridized with indicated probes. The decrease of dbx1, nkx6-1, nkx6-2 and evx1 is indicated by arrows. IS, injected side. (G-J) RT-PCR analysis of the expression of indicated genes in RA-treated neuralized animal caps isolated from embryos injected with mouse Prdm12 mRNA (50 or 250 pg/blastomere), amphioxus Prdm12 and chimeric constructs mRNA (250 pg/blastomere) as indicated. In G and H, expression levels were compared with the expression level of uninjected caps, defined as 1.

Image published in: Thélie A et al. (2015)

Copyright © 2015. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
en1.Len-1, en1-a, en1-b, eng1, engrailed 1, engrailed-1, xen1X. laevisThroughout NF stage 32spinal cord
evx1.Leve1, Xhox3X. laevisThroughout NF stage 32spinal cord
pax6.Lan2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6X. laevisThroughout NF stage 32spinal cord
dbx1.Sdbx, XdbxX. laevisThroughout NF stage 32spinal cord
nkx6-1.LLOC108704117, nkx6.1X. laevisThroughout NF stage 32spinal cord
nkx6-2.Lnkx6.2X. laevisThroughout NF stage 32spinal cord

Image source: Published

Experiment + Assay Source Phenotypes and Disease
Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.b
Expression Phenotype
decreased amount evx1.L expression in neural tube
Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.d
Expression Phenotype
decreased amount dbx1.S expression in neural tube
Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.e
Expression Phenotype
decreased amount nkx6-1.L expression in neural tube
Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.f
Expression Phenotype
decreased amount nkx6-2.L expression in neural tube

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