Xenbase Image ID: 151779

Fig. 4. Xenopus and mouse Prdm12, but not amphioxus Prdm12, downregulate dbx1, nkx6-1 and nkx6-2, and induces en1 in Xenopus. (A-F) Transverse sections at the neural tube of stage 32 embryos injected unilaterally with mPrdm12 mRNA (200 pg/blastomere) and hybridized with indicated probes. The decrease of dbx1, nkx6-1, nkx6-2 and evx1 is indicated by arrows. IS, injected side. (G-J) RT-PCR analysis of the expression of indicated genes in RA-treated neuralized animal caps isolated from embryos injected with mouse Prdm12 mRNA (50 or 250 pg/blastomere), amphioxus Prdm12 and chimeric constructs mRNA (250 pg/blastomere) as indicated. In G and H, expression levels were compared with the expression level of uninjected caps, defined as 1. Image published in: Thélie A et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue evx1.L eve1, Xhox3 X. laevis Throughout NF stage 32 spinal cord pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 32 spinal cord en1.L en-1, en1-a, en1-b, eng1, engrailed 1, engrailed-1, xen1 X. laevis Throughout NF stage 32 spinal cord nkx6-2.L nkx6.2 X. laevis Throughout NF stage 32 spinal cord nkx6-1.L nkx6.1 X. laevis Throughout NF stage 32 spinal cord dbx1.S dbx, Xdbx X. laevis Throughout NF stage 32 spinal cord Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.b Expression Phenotype decreased amount evx1.L expression in neural tube Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.d Expression Phenotype decreased amount dbx1.S expression in neural tube Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.e Expression Phenotype decreased amount nkx6-1.L expression in neural tube Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.4.f Expression Phenotype decreased amount nkx6-2.L expression in neural tube Larger Image Printer Friendly View

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