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Fig. 6. dbx1 and nkx6 overexpression blocks Prdm12 activity, and their knockdown upregulates En1. (A) RT-PCR analysis of en1 in neuralized animal caps overexpressing mouse Prdm12 mRNA (250 pg/blastomere), dbx1, dbx2, nkx6-1 or nkx6-2 mRNA (50 and 250 pg/blastomere) and treated with RA as indicated. (B) RT-PCR analysis of en1 and prdm12 in neuralized animal caps derived from embryos injected with MO against dbx1, nkx6-1 and nkx6-2 as indicated.

Image published in: Thélie A et al. (2015)

Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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