Xenbase Image ID: 151782

Fig. 7. RNA-Seq identification of Prdm12-regulated genes. (A) Experimental setup for the RNA-Seq analysis. (B) Selected ontological classification of genes regulated by Prdm12. (C) Examples of identified differentially expressed genes. (D) Venn diagram showing the overlap of differentially regulated genes between Prdm12, Prdm12-EnR and Prdm12-VP16. (E) Validation of some of the identified genes as prdm12 target genes by RT-PCR in RA-treated neuralized animal caps. (F) pax2 and gad1 on sections of embryos injected with either mouse Prdm12 mRNA (200 pg/blastomere) or with a Prdm12 MO. IS, injected side. (G) RT-qPCR analysis of pax2 and gad1 in EnR-Prdm12- and VP16-Prdm12-overexpressing RA-treated neuralized caps. Image published in: Thélie A et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 32 spinal cord gad1.1.L cpsq1, gad67 X. laevis Throughout NF stage 32 spinal cord Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Flag-Mmu.prdm12 + NF32 (in situ hybridization) Fig.7.f Expression Phenotype increased amount gad1.1.L expression in spinal cord increased amount pax2.L expression in spinal cord Xla Wt + prdm12 MO + NF32 (in situ hybridization) Fig.7.f Expression Phenotype decreased amount gad1.1.L expression in spinal cord decreased amount pax2.L expression in spinal cord Larger Image Printer Friendly View

Return to previous page