Xenbase Image ID: 151783

Fig. 8. Prdm12 binds dbx1 and nkx6 promoter regions, promotes their methylation and affects the activity of a dbx1 promoter-lacZ reporter construct. (A) IGV browser screenshots showing differential binding of Flag-mPrdm12 in RA-treated neuralized caps versus naïve caps in the promoters of dbx1, nxk6-1 and nkx6-2. Two major peaks in the dbx1 locus (at −3 kb and −5 kb), one in the nkx6-1 locus (at −0.5 kb), and another in the nkx6-2 locus (at −1.0 kb) are boxed. (B) ChIP-qPCR analysis of the dbx1, nkx6-1 and nkx6-2 promoter regions (with primers designed at the dashed boxes highlighted in A) in RA-treated neuralized caps overexpressing or not mPrdm12 with α-H3K9me2 (upper histogram) or α-H3K9me3 (lower histogram) antibodies. Fold enrichment is shown. (C) X-gal staining of a transverse section of the neural tube of a chick embryo electroporated with a 5.7 kb mouse eDbx1::lacZ reporter, plus or minus a pCIG-Prdm12 or an empty pCIG vector, and immunostained for GFP to show electroporation efficiency. Quantification of the number of X-gal+ cells observed in the electroporated side is shown (n≥4 embryos). Error bars denote s.e.m. **P<0.01. Image published in: Thélie A et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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