Xenbase Image ID: 151829

Fig. 2. Regulation of mesodermal and neural gene expression by Tbx6 in marginal zone explants. (A) Embryos at 4-cell stage were injected with Tbx6VP16 mRNA in the DMZ region or with Tbx6EnR mRNA in the VMZ region. Injected embryos were cultured to early gastrula stage for dissection of DMZ and VMZ explants. (B) Gene expression in DMZ and VMZ explants at the early gastrula stage. Tbx6 inhibits the expression of dorsal genes like Otx2 and induces the expression of ventral genes like Xwnt8 and szl in the DMZ explants. In contrast, Tbx6EnR induces the expression of dorsal genes like Otx2 in the VMZ explants, whereas it inhibits the expression of latero-ventral genes including Xbra, szl, Xwnt8, XMyoD and FGF8. (C) Gene expression in DMZ and VMZ explants expressing Tbx6VP16 or Tbx6EnR at early tail-bud stage. Tbx6 inhibits the expression of Otx2 and N-CAM, but up-regulates posterior genes Hoxb9, Xcad2 and Mespo in DMZ explants. Tbx6EnR induces the expression of neural markers XAG1, Otx2, En2 and N-CAM in VMZ explants, while it inhibits the expression of posterior genes including Hoxb9, Xcad2 and Mespo. Image published in: Li HY et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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