Xenbase Image ID: 151835

Fig. 8. Functional interaction between Tbx6, FGF8, Xwnt8 and XMyf5. (A–D) Interaction between Tbx6 and Xwnt8 in anteroposterior patterning. Embryos were injected at 4-cell stage and cultured to stage 35. (A) A control embryo. (B) dorsal(C) A Xwnt8Mo-injected embryo in the ventral region with enlarged head and shortened anteroposterior axis. (D) A Tbx6VP16-GR-injected embryo incubated in DEX at stage 11 shows anterior deficiency at stage 35. (E) Coinjection of Xwnt8Mo with Tbx6VP16-GR mRNA rescues head development. (F) Interaction between Tbx6, Xwnt8 and XMyf5 in myogenesis. Embryos were injected at 2-cell stage and animal cap explants were cultured to stage 11 for RT-PCR analysis. Inhibition of Xwnt8 and XMyf5 activity strongly inhibits XMyoD expression induced by Tbx6VP16. (G) FGF8 exhibits similar activity as Tbx6VP16 in inhibiting neural induction in explants cultured to stage 25. A dominant negative FGF receptor (XFD) inhibits XMyf5 and Xcad2 expression induced by Tbx6VP16 in explants cultured to stage 25. Image published in: Li HY et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + tbx6-VP16-GR + wnt8a MO + NF35/36 (whole-mount microscopy) Fig.8 B Anatomical Phenotype partially dorsalized embryo Xla Wt + tbx6-VP16-GR + wnt8a MO + NF35/36 (whole-mount microscopy) Fig.8 C Anatomical Phenotype decreased length of anterior-posterior axis increased size of the head Xla Wt + tbx6-VP16-GR + DEX + NF35/36 (whole-mount microscopy) Fig.8 D Anatomical Phenotype absent cement gland absent eye decreased size of the head Larger Image Printer Friendly View

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