XB-IMG-151890
Xenbase Image ID: 151890
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Fig. 9. Overexpression of a3 results in shortening along the antero-posterior axis and disruptions in somite segmentation. (A,B,E) Embryos derived from
control txa3 injected eggs. (C,D,F) Embryos derived from hxa3 injected eggs. (A,C) Whole-mount immunostaining with the somite-specific 12-101 antibody
(Kintner and Brockes, 1984). (B,D,E,F) Hematoxylin and eosin stained paraffin sections of injected embryos. Control injected embryos display a normal
pattern of somites (A,B) including well defined segments (E, arrowheads) and aligned myocyte nuclei (E, arrow). In contrast, hxa3 injected embryos are
shortened along the antero-posterior axis and have poorly defined somitic segments (C,D). Although myocyte nuclei are often observed in alignment (F,
arrow), segmental boundaries are not resolved in affected regions. The embryo in (D) shows a severe example of disruption of segmentation of the somitic
fields on both sides of the embryo. Note the appearance of a compact but relatively normal notochord in this embryo. e, ectoderm; s, somite; n, notochord.
The scale bar is 400 mm in (D) and 40 mm in (F). Image published in: Meng F et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |