XB-IMG-151920
Xenbase Image ID: 151920
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Figure 4
Smurf2 inhibits the biological functions of Smad1 in Xenopus embryo assays. (A) Alignments of the N-terminal amino acid sequences of Xenopus Smurf1 with Xenopus and human Smurf2. Identical amino acids are highlighted in black, and gaps are introduced to optimize the alignment. (B) Whole-mount in situ hybridization of xSmurf2 in Xenopus embryo. (Left) Gastrula stage; (Right) tailbud stage. (C) Effect of Smurf2 on marker gene expression. Animal poles of two-cell stage embryos were injected with 2 ng of mRNA of Smurf2 or Smurf2C716G (CG), 1 ng of Smad1 mRNA, 0.5 ng of Smad2 mRNA, or combinations of these as indicated. Animal caps were dissected at stage 9 and assayed at tadpole stage 32 by RT-PCR for the expression of the marker genes shown. (D) Comparison of the activities of Smurf2 and Smurf1. Two-cell stage embryos were injected with 2 ng of Smurf1 or Myc-Smurf2 mRNA, 1 ng of Smad1 mRNA, 0.5 ng of Smad2 mRNA plus 0.5 ng, 1 ng, or 2 ng of Smurf1 or Myc-Smurf2 mRNA. The two lanes on the far right in all panels are control reactions of total embryonic RNA, with (+) or without (−) reverse transcription. Image published in: Chang C et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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