XB-IMG-151926
Xenbase Image ID: 151926
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Fig. 1 Disruption of Smad1 consensus MAP kinase phosphorylation
sites by site-directed mutagenesis. (A) Consensus MAP kinase
phosphorylation sites within the linker domains of Smad
family members. Optimal sites (P-X-S/T-P) are shown in bold;
minimal sites (S/T-P) are underlined. Single sites are found in
the linker domains of Drosophila MAD, as well as vertebrate
Smads 2–4 and 6, although Xenopus and human Smad7 lack
consensus MAP kinase sites within the proline-rich linker region.
Four sites are present in Smads 1 and 5. (Sequences: Drosophila
MAD, Sekelsky et al., 1995; Xenopus Smad1, Thomsen, 1996;
human Smad1, Liu et al., 1996; Xenopus Smad2, Graff et al.,
1996; human Smad2, Lennon et al., 1996; human Smad3, Zhang
et al., 1996; rat Smad4, direct submission, GenBank accession
number AF056002; human Smad5, Riggins et al., 1996; Xenopus
Smad6, Nakayama et al., 1998a; human Smad6, Hata et al., 1998;
Xenopus Smad7, Nakayama et al., 1998b; human Smad7, Hayashi
et al., 1997). (B) Each of the four MAP kinase consensus
phosphorylation sites was altered by conversion of the phosphoacceptor
serine to alanine via two-step site-directed mutagenesis
(see Methods). Image published in: Sater AK et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |