Xenbase Image ID: 151928

Fig. 3 Loss of dorsal axial development in embryos expressing WT- or M4A-Smad1. Embryos were injected equatorially in two dorsal blastomeres at the four-cell stage with 1 ng mRNA encoding WT- or M4A-Smad1. When controls reached tailbud stages, embryos were fixed and immunostained with the 12/101 antibody. (A) Uninjected control showing labeled somites. (B) Embryos expressing WT-Smad1. In nearly all cases, small amounts of somitic tissue are present (arrows), even in the most ventralized embryos. (C) Embryos expressing M4ASmad1. 12/101 immunoreactivity is rarely detected in embryos ventralized by overexpression of M4A-Smad1 (n54 independent experiments). Image published in: Sater AK et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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