Xenbase Image ID: 151933

Fig. 8 Overexpression of M4A-Smad1 antagonizes neural specification in response to endogenous signals. (A) Embryos were microinjected with 500 pg/embryo of either WT-Smad1, M4ASmad1, or LacZ mRNA. Ectoderm from injected embryos was isolated at st. 10 and recombined with involuted dorsal mesoderm from mid-gastrula embryos. Recombinates were cultured until controls reached mid-neurula stages (15–16) and were harvested for RT-PCR. (B) Although otx2, NCAM, and N-tubulin are strongly expressed in recombinates of ectoderm expressing LacZ or WTSmad1, both otx2 and NCAM levels are reduced, and N-tubulin is not detected, in recombinates expressing M4A-Smad1 (n58 experiments). we, whole embryo (lane 1); Lac, recombinates of LacZ-expressing ectoderm (lane 2); mes, dorsal mesoderm only (lane 3); WT, recombinates of ectoderm overexpressing WT-Smad1 (lane 4); M4, recombinates of ectoderm expressing M4A-Smad1 (lane 5). Image published in: Sater AK et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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