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Fig. 1. Induction of Otx2 expression in ectodermal explants by IGF-I. (A) IGF pathway specifically activates Otx2 transcription. Synthetic mRNAs corresponding to Wnt-1, Dkk-1, chordin and IGF-I were injected near the animal pole region at 2-cell stage. Ectodermal explants from uninjected and injected embryos were dissected at blastula stage, explants from uninjected embryos were also treated with activin. RT-PCR was performed at early gastrula stage. Strong Otx2 expression was detected in explants expressing IGF-I, while activin, Wnt-1, chordin and Dkk-1 or a combination of chordin and Dkk1 had no effect on Otx2 expression. (B) Explants expressing indicated mRNA cultured to stage 25 express neural markers like Otx2 and N-CAM. (C) Specific inhibition of Xbra and Xwnt11 expression by IGF-I and Otx2 in activin-treated explants. IGF-I and Otx2 had no effect on the expression of chordin, goosecoid and Xwnt8 induced by activin Image published in: Carron C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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