Xenbase Image ID: 152081

Fig. 3. Cell lineage tracing of convergent extension movements during gastrulation in dorsally-injected embryos with IGF-I or Otx2 mRNA. (A) An embryo at stage 11 injected with Lac Z mRNA alone shows convergent extension of ß-gal-stained cells. (B) An IGF-I-injected embryo at the same stage shows delayed gastrulation and uniform distribution of ß-gal-stained cells in the dorsal region. (C) An Otx2-injected embryo with similar phenotype. (D) In an early neurula injected with Lac Z mRNA alone, ß-gal-stained cells are distributed in the narrowed trunk and posterior neural plate, indicating convergent extension movements. (E,F) Early neurula injected with IGF-I (E) or Otx2 (F) mRNA show open blastopore and uniform staining of ß-gal in the neural plate. (G) Longitudinal section of a stage 15 control embryo showing the elongation of neuroectoderm and mesoderm (mes). (H) Longitudinal section of an Otx2-injected stage 15 embryo with a reduced elongation of neuroectoderm and underlying mesoderm (mes). Image published in: Carron C et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + igf1 + NF11 (morphology) fig.3.b Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Xla Wt + otx2 + NF11 (morphology) fig.3.c Anatomical Phenotype abnormal gastrulation Xla Wt + igf1 + NF14 (morphology) fig.3.e Anatomical Phenotype abnormal gastrulation Xla Wt + otx2 + NF14 (morphology) fig.3.f Anatomical Phenotype abnormal gastrulation abnormally delayed closure of blastopore Xla Wt + otx2 + NF14 (morphology) fig.3.h Anatomical Phenotype abnormal convergent extension abnormal gastrulation Larger Image Printer Friendly View

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