XB-IMG-152090
Xenbase Image ID: 152090
|
Figure S2 (A) Real time PCR analysis with reverse transcription showing relative expression levels of ID2 messenger RNA in MDA-MB-231 cells transfected with Smad4 siRNA and Par1b siRNA#1 and treated with BMP2 (100 ng/ml for 2 hours) versus dorsomorphin. Induction of ID2 was attenuated upon Par1b depletion. (B) TGFβ induces pCAGA12-lux and this is not affected by inactivation of aPKCζ as tested with two independent dominant negative constructs. aPKCζII is a natural dominant negative of aPKCζ; aPKCζIIRD is derived from a deletion of aPKCζII and is an even more potent inhibitor of aPKCζII (1). Data are represented as mean and SD. (C) Quantitation of the wound healing assay of Figure 2F. (D) Representative pictures of embryo stained by situ hybridization for the BMP targets Sizzled and XVent-1. Embryos were injected at the 2-4 cell stage with control morpholino or Par1b (x+y) MO (as in Figure 2G and 2H), with and without BMP2 mRNA. Similar results were obtained with anti-Dvl2/3 morpholinos (data not shown). Image published in: Mamidi A et al. (2012) Copyright © 2012 Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license
Larger Image Printer Friendly View |