Xenbase Image ID: 1521

Figure 2. In situ hybridization for green fluorescent protein (GFP; left panels) and endogenous xflk-1 (right panels) in stage 20, 25, and 37 embryos. All embryos are shown with anterior to the left. Stage 20 embryos are shown from lateral (top) and ventral (bottom) views (top panels). Lateral view of stage 25 embryos (middle panels) and stage 37 embryos (bottom panel). At stage 20, both GFP and xflk-1 are detected in the presumptive endocardium (arrows). At swimming tadpole stages (stage 37), the expression pattern of the transgenic reporter (GFP) is similar to that of the endogenous xflk-1 gene. At earlier developmental stages, the GFP reporter is more highly expressed in the tail region of the embryo (arrowheads) than the endogenous xflk-1 gene. This finding is most obvious at stage 25 (center panels). Image published in: Doherty JR et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue kdr.L flk-1, flk1, LOC108698463, vegfr-2, vegfr2, xflk-1 X. laevis Throughout NF stage 20 heart primordium cardiac mesoderm kdr.L flk-1, flk1, LOC108698463, vegfr-2, vegfr2, xflk-1 X. laevis Throughout NF stage 25 tail region vascular endothelium posterior cardinal vein cardiac mesoderm kdr.L flk-1, flk1, LOC108698463, vegfr-2, vegfr2, xflk-1 X. laevis Throughout NF stage 37 and 38 heart tail bud vascular endothelium endocardium blood vessel head Image source: Published Larger Image Printer Friendly View

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