Xenbase Image ID: 152199

FIG 2. Anterior endomesoderm is specified by blastula stage. (A) Schematic diagram showing the experimental design. At the 4-cell stage, the dorsal blastomeres, which are lighter and smaller, are marked with Nile blue vital dye. At stage 7–8 the dorsal-vegetal tissues are explanted, free of mesoderm, and cultured until stage 10.5 when they are subjected to RT-PCR. (B) RT-PCR analysis of XHex, cerberus, XSox17Beta, gsc, Xbra, and Ef-1alpha expression in whole embryos stage 8 (WE st8) and stage 10.5 (WE st10.5), dorsal endoderm explants at stage 10.5 (DE), and a whole embryo stage 10.5 control cDNA with no reverse transcriptase (WE -RT). Mesoderm-free dorsal-vegetal explants that do not express Xbra do express XHex or cerberus. Image published in: Zorn AM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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