Xenbase Image ID: 152200

FIG. 3. Cell– cell signalling is required for anterior endomesoderm formation. (A) 4- to 8-cell-stage embryos were dissociated in Ca/Mg-free medium. Dissociated cells were maintained in a dispersed state by frequent agitation until sibling embryos reached stage 10.5 when the cells were harvested and subjected to RT-PCR. (B) A representative RT-PCR analysis is shown. XHex mRNA is not detected in dispersed cells (DissE), while cerberus and XSox17Beta expression is dramatically down-regulated. As control markers, Xbra expression has been shown to require cell contact, while Xwnt8 and gsc are expressed in a cell-autonomous manner. Stage 10.5 whole-embryo (WE) controls of cDNA with or without reverse transcriptase (+RT, -RT) are shown. Development of the AE requires communication between cells. Image published in: Zorn AM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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