XB-IMG-152202
Xenbase Image ID: 152202
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FIG. 5. Induction of XHex and cerberus in animal cap cells. (A) Synthetic mRNA was injected into the animal pole of 2-cell embryos. At stage 8–9 animal cap tissue was explanted and cultured until sibling embryos reached stage 10.5, when they were subjected to RT-PCR. (B) Embryos were injected with increasing doses of bVg1 mRNA (2, 20, 200 pg), siamois mRNA (10, 30, 100 pg), or noggin mRNA (10, 50, 200 pg). bVg1 induced XSox17, XHex and cerberus, while siamois induced only XHex expression. (C) Animal caps injected with XSox17 mRNA (500 pg) express cerberus but not XHex. (D) Animal caps injected with VegT/apod mRNA (500 pg) express XSox17 and cerberus, but not XHex. Stage 10.5 whole embryo (WE) controls of cDNA with or without reverse transcriptase (RT, RT) are shown. Image published in: Zorn AM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |