Xenbase Image ID: 152215

Supplemental Fig S2. Dorsal mesodermal injection (B1) of Sdc4-MOs or Sdc4/FZd7-MOs cause gastrulation defects. (A) Comparison of B1 (dorsal mesoderm) and D1 (anterior endoderm) injection of control MO (50 ng), Sdc4-MOs (50 ng) and Sdc4/Fzd7-MOs (25 ng each). In situ hybridization showing hhex expression at s2stage 11 and 19 after B1 or D1 injection of Sdc4/Fzd7-MOs (25 ng each). Image published in: Zhang Z et al. (2016) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Experiment + Assay Source Phenotypes and Disease Xla Wt + sdc4 MO + NF42 (whole-mount microscopy) Fig S2 A column 3 Anatomical Phenotype abnormal foregut morphology abnormal hindgut morphology decreased size of the eye obsolete abnormally edematous alimentary system Xla Wt + sdc4 MO + NF11 (immunohistochemistry) Fig S2 A column 4 Anatomical Phenotype abnormal gastrulation Xla Wt + sdc4 MO + NF19 (immunohistochemistry) Fig S2 A column 4 Anatomical Phenotype abnormal archenteron morphology abnormal gastrulation decreased size of the archenteron Xla Wt + sdc4 MO + NF35/36 (whole-mount microscopy) Fig S2 A column 4 Anatomical Phenotype dead whole organism Xla Wt + fzd7 MO + sdc4 MO + NF11 (immunohistochemistry) Fig S2 A column 5 Anatomical Phenotype abnormal gastrulation decreased size of the dorsal marginal zone Xla Wt + fzd7 MO + sdc4 MO + NF19 (immunohistochemistry) Fig S2 A column 5 Anatomical Phenotype abnormal archenteron morphology abnormal foregut endoderm morphology Xla Wt + fzd7 MO + sdc4 MO + NF42 (whole-mount microscopy) Fig S2 A column 5 Anatomical Phenotype abnormal alimentary system morphology abnormal foregut morphology abnormal hindgut morphology decreased size of the eye obsolete abnormally edematous alimentary system Xla Wt + fzd7 MO + sdc4 MO + NF11 (immunohistochemistry) Fig S2 A column 6 Anatomical Phenotype decreased size of the dorsal marginal zone Xla Wt + fzd7 MO + sdc4 MO + NF19 (immunohistochemistry) Fig S2 A column 6 Anatomical Phenotype abnormal endoderm morphology decreased size of the archenteron Xla Wt + fzd7 MO + sdc4 MO + NF19 (whole-mount microscopy) Fig S2 A column 6 Anatomical Phenotype abnormal gastrulation Xla Wt + fzd7 MO + sdc4 MO + NF35/36 (immunohistochemistry) Fig S2 A column 6 Anatomical Phenotype dead whole organism Larger Image Printer Friendly View

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