Xenbase Image ID: 152313

Figure S2. Phenocopy of tyr using Cas9 mRNA with another sgRNA in X. tropicalis founders and somatic mutation analysis using HMA. (A) Schematic of the genomic structure of the tyr gene and sgRNA targeting sites. Coding and untranslated exons are shown as yellow and cream coloured boxes, respectively. One (sgRNA1) was originally used in the main study, the other (sgRNA2) was made by shifting upstream from the original target site to confirm the resulting phenotypes. sgRNA target protospacer sequences are shown by pink letters and PAM sequences are highlighted in red boxes.(B) Disruption of tyr by sgRNA2 in founders. In vitro fertilisation (IVF) control embryos and representative phenotypes of tyr-disrupted embryos are shown (Asterisks). (C) Detection of targeted mutagenesis using HMA. Arrowheads and brackets indicate homoduplex and heteroduplex bands, respectively. Abbreviation; Wt, wild type. Image published in: Shigeta M et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xtr Wt + tyr CRISPR + NF41 (morphology) fig.S2.b Anatomical Phenotype absent eye decreased size of the eye increased size of the ventral trunk Larger Image Printer Friendly View

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