Xenbase Image ID: 152315

Fig. S1. Analysis of cell division in the proximal tubules of the pronephros. (A and B) 3G8 whole-mount immunostaining of uninjected controls and embryos injected with xDicer-morpholino oligomer (MO) at stage 45, demonstrating that the size of the pronephric tubules is still dramatically affected at late stages of kidney development. (C) Number of mitotic (pH3-positive) cells present in the proximal tubule of a single pronephros during stages 34–46 [equivalent of 45–106 h postfertilization (hpf)]. (D) Percentage of cell division was calculated for each stage by dividing the number of mitotic (pH3-positive) cells by the total number of cells for each individual embryo. Average and SDs of these data are depicted. Note that pH3-positive cells peak at stage 38, at a time point, when the number of total cells only starts to increase. Image published in: Romaker D et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + tsc1 MO + NF45 (immunohistochemistry) fig.S1.b Anatomical Phenotype abnormal proximal tubule morphology decreased size of the proximal tubule Larger Image Printer Friendly View

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