Xenbase Image ID: 152316

Fig. S2. Expression of Akt and S6 during Xenopus pronephros development. (A–J) Immunofluoresence analysis using antibodies recognizing all three Akt isoforms (Akt1/2/3) and the ribosomal protein S6. Representative sections of the pronephric area for stages 38–45 are shown. A and F are the secondary antibody only negative control. (K) Western blot analysis of whole embryo lysates comparing stages 34–45 using the same antibodies as used in the immunostainings. α-Actin marks equal loading control and asterisk indicates the position of pS6. Image published in: Romaker D et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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