XB-IMG-152386
Xenbase Image ID: 152386
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Fig. 1. Nuclear β-catenin is detected in premigratory NC cells, but not in migratory NC cells. A NC explants were dissected at premigratory stages and cultured in fibronectin-coated dishes. Explants were either fixed at premigratory stages (B, 30 min after explantation) or early (C, 3 h after explantation) or late migratory stages (D, 7 h after explantation). The β-catenin localization was determined by immunostaining (red). Upper panel shows β-catenin signal, lower panel includes DAPI staining. B β-catenin is predominantly localized in the nucleus and also at the plasma membrane in premigratory NC explants. C,D Early migratory and migratory NC explants show mainly membrane localization of β-catenin. A quantification of these results is shown in supplementary Fig. 6. E-K For in vivo analysis of canonical Wnt activity β-catenin expression was analyzed in transplanted GFP-expressing cells at premigratory NC stages (40 min after transplantation) or at tadpole stages (26–28). E Schematic representation of the transplanted embryos, the transverse section plane is indicated. F β-catenin is localized in the nucleus and at the plasma membrane in premigratory NC cells. F′ Higher magnification of the dashed area indicated in F. G,G′ shows the staining of the membrane-tethered GFP used to trace transplanted NC cells. H,H′ shows the merged β-catenin, GFP and DAPI staining. I β-catenin is localized exclusively at the plasma membrane of migrating NC cells. I′ Higher magnification of the dashed area indicated in I. J GFP staining of transplanted migrating NC cells. K, K′ Overlay of β-catenin, GFP and DAPI staining. Scale bars indicate 50 µm. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
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