XB-IMG-152387
Xenbase Image ID: 152387
|
Fig. 2. Activation of canonical Wnt signaling at premigratory and migratory NC cell stages affects NC migration. Wild type Xenopus embryos were treated with BIO at premigratory and migratory NC stages or with DMSO as a solvent control. NC migration was analyzed by Twist in situ hybridization. A The panel shows the different phenotypes observed after BIO treatment at premigratory stages. Phenotypes are categorized by decreasing severity: embryo with inhibited NC migration and reduced Twist expression (a), embryo with reduced NC branches (b), embryo with merged NC branches (c, arrow), unaffected embryo (d). B Graph summarizing the percentage of phenotypes observed in 5 independent experiments. Standard errors of the mean and number of treated embryos are indicated for each column. C The panel shows the different phenotypes observed after BIO treatment at migratory stages. Phenotypes are categorized by decreasing severity as for the premigratory treatment. Arrows mark reduced NC branches in (b) and merged branches in (c), unaffected embryo (d). D Graph summarizing the percentage of phenotypes observed in three independent experiments. Standard errors of the mean and number of treated embryos are indicated for each column. E,G Wild type Xenopus embryos were injected with 50 pg Lef1-GR RNA at the two-cell stage and treated with Dexamethasone at NC premigratory (E) and migratory stages (G). NC migration was analyzed by Twist in situ hybridization. E Panel shows the different phenotypes observed after Lef1-GR activation at premigratory stages: embryo with inhibited NC migration and reduced Twist expression (upper panel), embryo with reduced NC branches (lower panel). The injected side is shown on the right. F Graph summarizing the percentage of phenotypes observed in six independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. G The panel shows the different phenotypes observed after Lef1-GR activation at migratory stages. The injected side of the embryo is shown on the right. Phenotypes are categorized as in the premigratory treatment. H The graph summarizes nine independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published Larger Image Printer Friendly View |