XB-IMG-152388
Xenbase Image ID: 152388
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Fig. 3. Inhibition of canonical Wnt signaling at premigratory and migratory NC cell stages affects NC migration. Wild type Xenopus embryos were treated with IWR1 at NC premigratory and migratory stages. DMSO treatment served as a solvent control. NC migration was analyzed by Twist in situ hybridization. A The panel shows an embryo with merged NC branches (right panel, arrow), the phenotype observed after IWR1 treatment at premigratory stages. An untreated control embryo is shown on the left. B Graph summarizing the percentage of phenotypes of four independent experiments. Standard errors of the mean and number of treated embryos are indicated for each column. C Embryo with merged NC branches (right panel, arrow) after IWR1 treatment at migratory stages. A control embryo is shown on the left. D The graph summarizes three independent experiments. Standard errors of the mean and number of treated embryos are indicated for each column. E Wild type Xenopus embryos were injected with Tcf3∆C-GR RNA in one blastomere at the two-cell stage and embryos were treated with Dexamethasone at NC premigratory and migratory stages. NC migration was analyzed by Twist in situ hybridization. The panel shows the different phenotypes observed after Tcf3∆C-GR activation at premigratory stages: embryo with inhibited NC migration and reduced Twist expression (upper panel), embryo with reduced NC branches (lower panel). The injected side of the embryos is shown on the right. F Graph summarizing the percentage of phenotypes in 9 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. G The panel shows the different phenotypes observed after Tcf3∆C-GR activation at migratory stages. The same phenotypes as for the premigratory Dexamethasone treatment are observed. The injected side of the embryo is shown on the right. H Graph summarizing three independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
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