XB-IMG-152393
Xenbase Image ID: 152393
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Supplementary material Supplementary Fig. 1 Activation of canonical Wnt signaling affects the migration of Sox10-positive NC cells. A-D Wild type Xenopus embryos were treated with BIO or IWR1 at premigratory NC stages and DMSO treatment served as a solvent control. NC migration was analyzed by Sox10 in situ hybridization. A The panel shows the different phenotypes observed after BIO treatment at premigratory stages. Phenotypes are categorized by decreasing severity: embryo with inhibited NC migration (a), embryo with merged NC branches (b), unaffected embryo (c). Arrow marks cells with inhibited migration; merged NC cells are indicated by a dashed line. B Graph summarizing 4 independent experiments. Standard errors of the mean and number of treated embryos are indicated for each column. C The panel shows the different phenotypes observed after IWR1 treatment at premigratory stages. Panel shows the ‘inhibited migration’ phenotype (a) or an unaffected embryo (b). D Graph summarizes 4 independent experiments for 10 µM and 20 µM of IWR1 and 3 independent experiments for 100 µM of IWR1. Standard errors of the mean and number of injected embryos are indicated for each column. E Wild type Xenopus embryos were injected with 50 pg of Lef1-GR RNA in one blastomere at the 2-cell stage and treated with Dexamethasone at NC premigratory stages. NC migration was analyzed by Sox10 in situ hybridization. The panel shows the different phenotypes observed after Lef1-GR activation at premigratory stages: embryo with a strong inhibition of NC migration (upper panel), embryo with merged NC branches (lower panel). Injected side of the embryo is shown on the right; arrows marks NC cluster in the premigratory position; merged NC cells are indicated by a dashed line. F Graph summarizing 5 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. G Wild type Xenopus embryos were injected with Tcf3∆C-GR RNA in one blastomere at the 2-cell stage and treated with Dexamethasone at NC premigratory stages. NC migration was analyzed by Sox10 in situ hybridization. The panel shows the different phenotypes observed after Tcf3∆C-GR activation at premigratory stages: embryo with inhibited NC migration (upper panel), embryo with merged NC branches (lower panel). Injected side of the embryo is shown on the right. Arrow marks NC cluster in the premigratory position and the remaining mandibular branch; merged NC cells are indicated by a dashed line. H Graph summarizing 5 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
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