XB-IMG-152396
Xenbase Image ID: 152396
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Supplementary material Supplement Fig. 4 Activation of canonical Wnt signaling at premigratory and migratory stages in whole embryos by overexpressing a Dexamethasone-inducible construct of Tcf3-VP16-GR. Wild type Xenopus embryos were injected with 50 pg Tcf3-VP16-GR RNA at the 2-cell stage and treated with Dexamethasone at NC premigratory and migratory stages. NC migration was analyzed by Twist in situ hybridization. A Embryo with reduced NC branches (arrow), which is the predominant phenotype observed after Tcf3-VP16-GR activation at premigratory stages. The injected side is shown on the right. B Graph summarizing 5 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. C Embryo with reduced NC branches (arrow) representing the phenotype observed after Tcf3-VP16-GR activation at migratory stages. Injected side is shown on the right. D The graph summarizes 5 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. E Wild type Xenopus embryos were injected with 50 pg of Tcf3-VP16-GR RNA in one blastomere at the 2-cell stage and treated with Dexamethasone at NC premigratory stages. NC migration was analyzed by Sox10 in situ hybridization. The panel shows an embryo with merged NC branches, which is the predominant phenotype observed after Tcf3-VP16-GR activation at premigratory stages. Injected side of the embryo is shown on the right. F Graph summarizing 6 independent experiments. Standard errors of the mean and number of injected embryos are indicated for each column. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
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