XB-IMG-152398
Xenbase Image ID: 152398
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Supplementary material Supplementary Fig. 6 The levels of nuclear β-catenin change during stages of NC migration and are upregulated by BIO treatment. NC explants were dissected at premigratory stages and fixed at premigratory stages (30 min after explantation) or migratory stages (3 h or 7 h after explantation). β-catenin localization was determined by immunostaining. A β-catenin is predominantly localized in the nucleus and also at the plasma membrane in premigratory NC explants. B,C Early migratory and migratory NC explants show mainly membrane localization of β-catenin. D,E NC explants treated with 1 µM BIO for 3 h (D) or 7 h (E) showing translocation of β-catenin into the nuclei and elevated levels of cytoplasmic β-catenin. F Graph summarizing averaged fluorescent intensity expressed as Integrated Density of control and BIO-treated explants. The area of each nucleus was determined by DAPI staining and the intensity of the β-catenin fluorescent signal expressed as Integrated Density was measured using ImageJ. Integrated Densities of β-catenin fluorescent signals from all explants of a particular treatment were averaged and normalized to the premigratory controls. One Way ANOVA and post hoc Games-Howell test were used for statistical analysis. Numbers of nuclei and explants analyzed are indicated for each column. BIO treatment leads to increased nuclear β-catenin compared to controls. One Way ANOVA and post hoc Games-Howell test confirm that NC explants treated with 1 µM BIO for 3 h (D) show similar levels of nuclear β-catenin compared to untreated NC explants fixed 30 min after explantation (A). The scale bar resembles 50 µm. Image published in: Maj E et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
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