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Fig. 4. Analysis of the genes only identified by the exon-centric approach. A, We manually classified the 189 genes with one or more differential exon, but without any differential junction, into 5 classes: RI (retained intron), 3CPA (differential exon 3′ to a cleavage and polyadenylation site), SJS (supported by a junction in Sashimi plots), VWE (very weakly expressed) and NSJS (non-supported by a junction in Sashimi plots). The pie chart shows the distribution of the genes between these 5 classes. B–E, Sashimi plots of representative genes within each of 4 classes (no gene belonging to the RI class is shown since this class only contains two members). The orientations of the gene are given by the arrows. The genomic regions identified as a differential exon are circled dotted red. F, Boxplot of the number of reads per gene, for genes identified with the exon-centric approach only and genes identified with both the exon-centric and the all-junctions-centric approaches.

Image published in: Noiret M et al. (2017)

Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.

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