Xenbase Image ID: 152441

Fig. 1. Siamois and Twin bind an identical conserved region within the Gsc proximal element. (A) Schematic of the Gsc promoter indicating sequence conservation within the proximal element (PE) across species. The P3 element and upstream half site are indicated by gray shading. DNase footprinting was performed on the Gsc promoter to identify regions protected by Sia and Twn (B,C). A double-stranded fragment of the Gsc promoter, radiolabeled on the top (B) or bottom (C) strand, was incubated with full-length Sia, Sia homeodomain (Sia HD), Twn homeodomain (TwnHD) or a mixture of the Sia and Twn homeodomains (S + T HD). Protected regions are indicated to the right of each autoradiogram (B,C) and summarized in schematic form (D). In addition to the major protected region containing the P3 site and the upstream half site, two minor protected regions (− 103 to − 93 and -15 bp to + 1 bp) were detected as well, but these did not contain apparent homeodomain binding sites and may be either non-specific or cryptic homeodomain binding sites. The region of protection for the top strand is overlined and for the bottom strand is underlined in (D), with the upstream half site and the P3 site indicated by gray shading. GA indicates a sequencing reaction run with purine terminators, providing a size ladder for DNAse cleavage products. Image published in: Bae S et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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