Xenbase Image ID: 152444

Fig. 4. Siamois and Twin homodimers and heterodimers occupy the endogenous Gsc promoter. (A) Genomic regions recovered by chromatin immunoprecipitation for myc-Sia, myc-Twn or myc-SiaQ191E were evaluated by quantitative PCR (QPCR) for either the Gsc promoter or EF1α locus as control. The mean fold enrichment (normalized to uninjected samples) and standard error for three independent experiments are presented. (B) Genomic regions recovered by sequential chromatin immunoprecipitation were evaluated by QPCR for the Gsc promoter or Xmlc2 locus as control. Differentially tagged forms of Sia and Twn were coexpressed, samples were subjected to two rounds of immunoprecipitation, and recovered genomic sequences were analyzed by QPCR for each round. Coinjected mRNAs are indicated for myc-Sia, myc-Twn, GST-Sia and GST-Twn, and the order of the myc and GST immunoprecipitations are indicated as 1st IP and 2nd IP. As a control, a first immunoprecipitation with myc-Sia and a second immunoprecipitation with GST alone was also performed. Neither the Gsc nor Xmlc genomic regions were significantly recovered from the second immunoprecipitation. The mean fold enrichment (normalized to uninjected samples) and standard error for five independent experiments in presented. Image published in: Bae S et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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