Xenbase Image ID: 152448

Supplementary Fig. 1. The Siamois or Twin homeodomain is sufficient for DNA-binding and complex formation at the Gsc proximal element. Increasing amounts of purified Sia homeodomain (A) or Twn homeodomain (B) was incubated with the indicated radiolabeled EMSA probes. Probe sequences for wild-type and mutated forms of the Gsc proximal element are shown in Fig. 2A. Monomer (M) and dimer (D) complexes were observed for the WT, 136 MT and 127 MT probes. The monomer complex only was observed for the 2X MT probe and no complex formation was observed for 3X MT (data not shown). F, free probe. Image published in: Bae S et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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