Xenbase Image ID: 152488

Figure 4. H+/K+-ATPase mRNA in Xenopus and Chick Embryos (A) H+/K+-ATPase α mRNA is localized preferentially to the animal hemisphere of the unfertilized Xenopus egg (shown in histological section). (B and C) Animal views showing asymmetric localization patterns seen occasionally at the 2-cell stage (B) and consistently in the 4-cell stage. (D–F) Histological sections through animal hemisphere of 4-cell embryos confirm that the mRNA localization is preferentially localized within right ventral blastomeres (red arrowheads) and depleted from the left ventral blastomere (white arrowheads), but is symmetric in the smaller dorsal blastomeres. Dorsal is oriented to the top in (C–F). (G) Dorsal view of an 8-cell embryo showing LR symmetrical expression in the dorsal animal blastomeres and the narrow band of mRNA on the animal side of the dorsovegetal blastomeres (arrowhead). (H) After MBT (stage 8), H+/K+-ATPase α mRNA is expressed symmetrically throughout the animal cap, as visualized in transverse histological section. (I) H+/K+-ATPase α mRNA is localized in neural tissues and posterior gut of tailbud stage (stage 32) embryos. (J and K) HH stage 3+ chick embryos showing symmetrical expression of H+/K+-ATPase α and β subunit mRNAs. The mRNAs remain symmetrically expressed in the ridges of the primitive streak at all stages examined (HH stages 2+ to 4−). Image published in: Levin M et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue atp4a.L alpha H-K, gastric H(+)-K(+)-ATPase alpha-subunit, LOC108696872, MGC69393 X. laevis Sometime during NF stage 4 (8-cell) to NF stage 32   Image source: Published Larger Image Printer Friendly View

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