XB-IMG-152490
Xenbase Image ID: 152490
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Figure 6.
LR Asymmetries of Membrane Potential Patterns in the Primitive Streak Area Are Modulated by BaCl2 or Omeprazole
(A–D) A transient domain of depolarization to the left side of the primitive streak in chick embryos visualized with the potentiometric fluorescent probe DiBAC4(3). The blue to green to red pseudo-color scale represents increasing fluorescence intensities reflecting increased accumulation of the anionic dye in intracellular membranes. Increased fluorescence corresponds to a less negative membrane potential (i.e., depolarized).
(E–H) Linescans of the embryos made perpendicular to the streak (red lines in A-D) show the increased left sided fluorescence. Supplemental Table S3, available at http://www.cell.com/cgi/content/full/111/1/77/DC1, summarizes the mean LR differences from all embryos tested.
(I–K) Typical LR asymmetric pattern of depolarization is seen prior to exposure (I). The streak is outlined in (I). A relative increase in right-sided fluorescence is seen in the same embryo as in (I) after exposure to BaCl2 (J). Similar increase in right-sided fluorescence after omeprazole exposure (K). Arrowheads indicate Hensen's node.
(L–N) Linescans of the embryos (made along the red lines in I–K).
Image published in: Levin M et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |